Protein Biophysics

Course goals

Course aims and outcomes:

1. Develop deep understanding of protein biophysics, theory and hands on experience of protein characterisation
2. Familiarise with available techniques to characterise proteins with critical understanding of advantages and disadvantages of each
3. Develop the skills to design and execute experiments to analyse proteins concentration, size, stability and aggregation.
4. Develop the skills to design and execute experiments to label proteins and analyse biomolecular interactions.
5. Practice writing a short manuscript to report lab results

Content

Course description:
This course aims in providing deep understanding of protein biophysics with an emphasis on hands-on experience making use of the instrumentation in the protein research centre: https://www.uu.nl/en/research/protein-research-centre
Topics covered in the course includes: protein biophysics (size, stability and aggregation); Biomolecular interactions; Protein labelling.
The students will learn about the following methods and technologies: fluorescence anisotropy, size exclusion chromatography, microscale thermophoresis (MST), Flow Induced Dispersion Analysis (FIDA), dynamic light scattering (DLS) and nano differential scanning fluorimetry (nDSF)

Tests
summative
20% presentation
20% report
60% exam